Koushik Shah, Ph.D. — Operations Associate

An inquisitive Protein Biologist with cumulative 9 years of experience in Protein-DNA binding assay and Protein-Protein interactions, Method development, Proteomics, Molecular Biology, gene regulation and epigenetics. As a post-doctoral scientist, I worked on regulatory monomeric and dimeric transcription factors binding, their structural insights, and heterodimeric functions with DNA inside the cell. During PhD, identified potential target DNA binding sites of bZIP TFs in hexaploid plant using biochemical and in-vitro binding analysis combined with NGS. Proficient in working with multidisciplinary teams and have inquisitive mind to solve scientific problems. Bring good communication skills and work ethics with mutual respect towards work space. Currently, working as Assistant Manager (R&D) in living therapeutic division at Micro Crispr Private Limited (MCPL)- Meril. In this new role, I am precisely contributing to the development of cutting-edge Monoclonal and Recombinant antibody technologies, driving innovation in diagnostics and therapeutics for various treatment or drug therapies. Skills and Techniques: * Plasmid construction and transformation, vector engineering, High-throughput cloning techniques (SLIC,TA,Gibbson etc.),PCR and qRT-PCR variant, site-directed mutagenesis,PCR purification, primer designing and screening of clones in cost effective manner. * Cloning of ORFs and their expression, protein engineering, purification of recombinant proteins in high-throughput way using bacterial and animal cell systems. * Assay and method development for protein-DNA binding, Peptide modification and protein-protein interaction invivo and in-vitro system. * DNA-protein binding,aptamers specificity and affinity analysis using ChIP-seq, SELEX, DAP-seq. * Characterization and purification of proteins using techniques like analytical HPLC(C8 & C18),SDS-PAGE, Western Blot, dialysis, affinity,ion-exchange and size-exclusion chromatography (SEC). * Expertise in DNA and RNA analysis, DNA sequencing, RNA-seq, genomics and next generation sequencing (NGS) Miseq platform, Library preparation, Sonication, DNase treatment and purification. * Working expertise on handling biophysical techniques such as Ultracentrifugation(Beckman C.), Isothermal calorimetry (ITC), Thermal-shift assay (TSA), Circular Dichroism(CD) spectroscopy, UV-Vis Spec, EMSA (gel-shift assay), Fluorescence anisotropy, nano LC-MS/MS, MALDI-TOF, Mass spectrometry(ABsciex) analysis and Gel electrophoresis.

Stackforce AI infers this person is a Protein Biologist specializing in Antibody Engineering within the Biotechnology industry.

Location: Vapi, Gujarat, India

Experience: 12 yrs 6 mos