Krishna Mohan Poluri, FRSC

CEO

Roorkee, Uttarakhand, India17 yrs 5 mos experience

Most Likely To SwitchHighly Stable

Key Highlights

Expert in Structural and Computational Biology.
Extensive experience in Biophysical Chemistry and Molecular Biology.
Proven track record in RNA polymerase functionality research.

Stackforce AI infers this person is a leading expert in Biophysical and Molecular Biology research.

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Skills

Core Skills

Molecular BiologyBiophysical Chemistry

Other Skills

RNA polymerasegene expressionregulationdynamic propertiesstructural and dynamic dataBiophysicsProtein ChemistryProtein ExpressionNMR spectroscopyBiochemistryImmunologyrecombinant DNA technologyGlycobiologyStructural BiologyProtein Engineering

About

Specialties: Structural and Computational Biology: *Multi Dimensional Nuclear Magnetic Resonance Spectroscopy *Algal Biotechnology - Bio-remediation & Biofuels *Biomedical & Environmental Nanotechnology Biophysical, Computational & Molecular Biology Techniques *Protein Expression & Purification *Selective Labeling of Proteins *Gene Cloning & Mutagenesis *Fluorescence Spectroscopy & Fluorescence Microscopy *Isothermal Calorimetry, Circular Dichroism, *Molecular docking and Simulations

Experience

17 yrs 5 mos

Total Experience

4 yrs 4 mos

Average Tenure

12 yrs 9 mos

Current Experience

Indian institute of technology, roorkee

3 roles

Professor

Promoted

Oct 2023 – Present · 2 yrs 7 mos

Associate Professor

Promoted

Dec 2018 – Oct 2023 · 4 yrs 10 mos

Assistant Professor

Aug 2013 – Dec 2018 · 5 yrs 4 mos

Utmb

Research Scientist

Jan 2012 – Aug 2013 · 1 yr 7 mos

Rutgers university

Post Doctoral Associate

Feb 2009 – Dec 2011 · 2 yrs 10 mos

RNA polymerase (RNAP), the principal enzyme of gene expression and regulation. RNAP is conserved in all living organisms. RNAP binds to specific promoter sequences, and synthesizes the RNA chain complementary to the template strand.
We are addressing central questions regarding the mechanisms underpinning RNAP functionality. More specifically, we will determine the intrinsic dynamic properties of the enzyme (opening/closing of various channels and RNAP ‘breathing’) and how the dynamics is regulated by promoter and terminator sequences, formation of the initiation complex, formation of the elongation complex and RNA synthesis. We also intend to study the mechanisms of active-center conformational cycling using an integrated biophysical approach aiming at obtaining simultaneously structural and dynamic data of different states of RNAP (closed, open and elongation complex). This current work will shed light into central questions regarding the fundamental mechanisms underpinning the RNAP functionality.

RNA polymerasegene expressionregulationdynamic propertiesstructural and dynamic dataMolecular Biology+1