Feb 2023 – Oct 2023 · 8 mos · Chicago, Illinois, United States · On-site

• Strong skills for working effectively within CAP/CLIA-regulated labs.
• Possess the ability to autonomously address equipment-related challenges during critical operational periods, spanning from sample super-pooling to sequencer loading, thereby contributing to enhanced economic efficiency.
• My responsibilities also extend to drafting, reviewing, and maintaining SOPs, batch record templates, and other quality-related documentation for Tempus's NGS labs.
• Skilled in operating liquid handlers and various automation platforms for efficient sample processing.
• Proficient in processing, passaging, and cryopreserving tissue organoids.
• Experienced in utilizing digital notebooks such as LIMS and gLIIS.
• Skilled in hands-on sample processing and loading onto genome sequencers, coupled with expertise in analyzing resulting data using computational tools.

Oct 2022 – Jan 2023 · 3 mos · Evanston, Illinois, United States

• Worked with a multidisciplinary team to strategize, design, and develop optimized experimental protocols to express and purify recombinant from of human PDE enzyme from bacteria achieving higher yields and minimizing costs.
• Led efforts to optimize expression and purification parameters of PDE, resulting in improved enzymatic activity.
• Took leadership in troubleshooting experimental failures and coordinated with the team for possible suggestions.

Sep 2020 – Sep 2020 · 0 mo · Seoul, South Korea

• •Acted as Ali's wife in the Korean drama series "Squid Game" (Episode 2).

Sep 2017 – Aug 2023 · 5 yrs 11 mos

• Strategically planned objectives and devised experimental steps to achieve successful recombinant Butelase-1 expression. My efforts resulted in the successful filing of a Korean patent application for reporting the highest yield through the bacterial host, demonstrating my ability to strategically plan and execute complex research projects.
• Developed and a cost-effective and simple enzyme-based assay detecting protein cyclization significantly reducing analytical expenses compared to traditional MS methods.
• Consistently maintained optimal storage conditions for bacterial cultures and plasmids, guaranteeing their availability for ongoing research.
• Employed bacterial hosts to successfully cyclize large and complex proteins, like HRP, while subsequently validating product outcomes using our internally devised enzyme-based assay technique.
• Effectively communicated with vendors, streamlined purchase order processes, and maintained a well-organized inventory to minimize delays in research due to material shortages.
• Authored and co-authored peer-reviewed scientific publications.

Sep 2017 – Aug 2022 · 4 yrs 11 mos

• 1. Gene cloning, expression optimization, and purification of a recombinant cyclase.
• 2. Development of a facile assay for the detection of protein cyclization.

May 2015 – Apr 2017 · 1 yr 11 mos · Guwahati, Assam, India

• Drove cost-effective gains by successfully executing recombinant expression and purifying recombinant Spo0A in E. coli via optimized protein expression conditions and efficient chromatographic techniques.
• Improved TAG biosynthesis—a biodiesel precursor, by leveraging CRISPR-Cas9 technology in Rhodococcus opacus.
• Orchestrated an economic upswing by proficiently scaling up and managing a bench-scale bioreactor (3L), significantly elevating TAG production levels.

Aug 2013 – Feb 2014 · 6 mos · Kolkata, West Bengal, India

• • Conducted comprehensive structural analysis and characterization of the cytochrome c protein through fundamental bioinformatics tools.